Viral agents associated with poult enteritis in Croatian commercial turkey flocks
2010
Lojkic, I.,Croatian Veterinary Inst., Zagreb (Croatia). Dept. of Virology | Bidin, M.,University of Zagreb (Croatia). Dept. of Poultry Diseases | Bidin, Z.,University of Zagreb (Croatia). Dept. of Poultry Diseases | Mikec, M.,Croatian Veterinary Inst., Zagreb (Croatia). Poultry Centre
From 2003 to 2006, samples of intestinal content and spleens from 10-day-old to 6-week-old fattening turkeys showing clinical signs of enteritis were analyzed by specific PCR and RT-PCRs for detection of haemorrhagic enteritis virus (HEV), avian reovirus (ARV), turkey astrovirus-2 (TastV-2), and turkey coronavirus (TCV). A total of 23 flocks from 6 farms were included in the study. Specific sequence for HEV hexon gene was present in 7 samples. A product of TastV-2 capsid gene was detected in 17/23 intestinal content samples. A 626-bp band of sigma A encoding gene segment from avian reovirus was present in three samples, all from the same farm. Sequence analysis of 450 bp fragment of avian reovirus sigma A encoding gene sequence showed that our strain had the identity of 91.3% with the strains 138, 2408, 1733, 919, T6, and Os161. No TCV specific PCR band was found in any sample. Four flocks were positive simultaneously for HEV and TastV-2, and three flocks on TastV-2 and ARV. Severity of poult enteritis described in our study is caused by immunosuppressive TastV-2 in combination with HEV or ARV.
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