Concurrent occurrence of alpha-amylase inhibitor and stimulator in red kidney bean seed: physiological implications
2010
Alizadeh, H.,University of Canterbury, Christchurch (New Zealand). School of Biological Sciences | Leung, D.W.M.,University of Canterbury, Christchurch (New Zealand). School of Biological Sciences | Cole, A.L.J.,University of Canterbury, Christchurch (New Zealand). School of Biological Sciences
It is hypothesized that since protein alpha-amylase inhibitor (alpha-AI) and stimulator might be present together in red kidney bean seeds, their in vitro interactions might influence their detection and quantification. Assay of alpha-AI using extracts from the embryonic axes revealed an unexpected finding in that the extracts stimulated rather than inhibited alpha-amylase activity. The cotyledon extracts exhibited inhibitory or enhancement effect on alpha-amylase activity depending on whether prior to the alpha-amylase assay they had been boiled for 10 min or not. Phytohemagglutinin (PHA-L in particular) is implicated in the present study as a stimulator of alpha-amylase activity co-extracted with alpha-AI from red kidney bean cotyledons. The importance of these findings is discussed in relation to the possible widespread occurrence of protein alpha-amylase stimulator in seeds and other plant parts.
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