Cloning of phenazine carboxylic acid genes of Fusarium moniliforme antagonists bacteria in Escherichia coli DH5alpha
2009
Niknejad Kazempour, M.,University of Guilan, Rasht (Iran Islamic Republic). Dept. of Plant Pathology | Anvary, M.,University of Guilan, Rasht (Iran Islamic Republic). Dept. of Plant Pathology
Bakanae disease caused by Fusarium moniliforme is an important fungal disease of rice. Pseudomonas fluorescens produces a broad-spectrum antibiotic, phenazine-carboxylic acid (PCA), which is active against a variety of fungal root pathogens. In this study contaminated rice samples were collected from infected farms of Guilan. Moreover, 238 bacteria were isolated from rhizosphere. The antagonistic ability of 12 of them was demonstrated with two culture methods. According to biochemical and culturing trial results, 8 isolates were identified as Pseudomonas fluorescens. The effects of volatile HCN compounds produced by P. fluorescens were studied. All isolates inhibited the growth of F. moniliforme in vitro. DNA of all isolates was extracted by the CTAB method and phenazine C and D genes were cloned in E. coli DH5alpha by standard methods. The successful cloning was confirmed by the blue/white screening results and recombinant plasmid agarose gel electrophoresis. These results suggest that P. fluorescens that produce PCA might play an important role in the natural suppressiveness of some soils to the causal agent of collar and root rot of rice.
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