Genetic characterization of Pseudomonas syringae pv. syringae strains from stone fruits based on RAPD analysis in Iran
2009
Khayamie, S.,University of Guilan, Rasht (Iran Islamic Republic). Faculty of Agriculture | Niknejad Kazempour, N.,University of Guilan, Rasht (Iran Islamic Republic). Faculty of Agriculture | Rabie, B.,University of Guilan, Rasht (Iran Islamic Republic). Faculty of Agriculture | Sassanie, S.,University of Guilan, Rasht (Iran Islamic Republic). Faculty of Agriculture | Ebadie, A.A.,University of Guilan, Rasht (Iran Islamic Republic). Faculty of Agriculture
Bacterial canker is caused by the bacterial pathogen Pseudomonas syringae pv. syringae which is present in Iran. Random amplified polymorphic DNA (RAPD) was used to assess the genetic structure of P. syringae pv. syringe populations. In this research, genetic diversity of 50 P. syringae pv. syringae isolates from stone fruit trees, olive and hazelnut were analyzed. Out of 100 bands, generated with 8 RAPD primers, 72% were polymorphic and scored as molecular markers. Nei-Li similarity coefficients were computed for every pair of strains from data on fragment presence or absence. Relatedness between strains was evaluated by UPGMA clustering. The results of genetic analysis showed that hazelnut isolates are completely separated from stone fruit isolates. In addition, all of the stone fruit isolates were divided in 3 clusters by RAPD primers. This study shows that strains of P. syringae pv. syringe exhibit genetic diversity detectable by RAPD analysis, and that molecular and statistical analysis of RAPD fragments can be used both to distinguish between strains and to determine relatedness between them.
Показать больше [+] Меньше [-]Ключевые слова АГРОВОК
Библиографическая информация
Эту запись предоставил Library of Antonin Svehla