Detection and analysis of two epistatic quantitative trait loci on SSC12 affecting litter size in pigs | Análisis de dos QTL epistáticos en el cromosoma 12 porcino para el carácter tamaño de camada
2009
Fernández Rodríguez, A., Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria, Madrid (España). Dept. de Mejora Genética Animal | Rodríguez, M.C., Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria, Madrid (España). Dept. de Mejora Genética Animal | Noguera, J.L., Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria, Madrid (España). Dept. de Mejora Genética Animal | Balcells, I., Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria, Madrid (España). Dept. de Mejora Genética Animal | Ovilo, C., Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria, Madrid (España). Dept. de Mejora Genética Animal | Fernández, A.I., Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria, Madrid (España). Dept. de Mejora Genética Animal
The aim of the present study was to confirm the previously detected epistatic QTL on SSC12 for total number of piglets born (TNB) and number of piglets born alive (NBA) in a Meishan x Iberian F2 intercross. A chromosome scan with 12 markers allowed us to confirm the two epistatic QTL (QTL1 at 15cM and QTL2 at 97cM). Besides, SLC9A3R1 and NOS2a were analysed as biological and positional candidate genes underlying these QTL. Both cDNAs were characterized and 23 polymorphisms were identified in the F2. Five polymorphisms were tested: two in SLC9A3R1 for QTL1 and four in NOS2a for QTL2. Only SLC9A3R1c.1359A>G reported significant effects on TNB and NBA, while the three SNPs of NOS2a showed significant additive effects on both traits. Marker assisted association test performed with the most significant SNPs (SLC9A3R1 c.1359A>G and NOS2a c.2192C>T) reported significant results for both SNPs; however the interaction between them was not significant. Despite of the negative result of the interaction, statistical and biological evidences show NOS2a c.3556_3571dupCTGTATTTACCTGAC, cosegregating with c.2192C>T, as a likely causal mutation of the epistatic effect.
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