Genetic variability in the acetyl-CoA carboxylase gene in Churra and Ojalada sheep breeds | Estudio de la variabilidad del gen de la acetil-CoA carboxilasa alfa (ACACA) en las razas ovinas Churra y Ojalada
2009
García Fernández, M., Universidad de León (España). Facultad de Veterinaria | Gutiérrez Gil, B., Universidad de León (España). Facultad de Veterinaria | García Gámez, E., Universidad de León (España). Facultad de Veterinaria | Arranz, J.J., Universidad de León (España). Facultad de Veterinaria
Acetyl-CoA carboxylase (ACACA) is the rate-limiting enzyme in the biosynthesis of long-chain fatty acids. We have sequenced about 4.6 kb of the ovine ACACA cDNA, which included partial exon 1 to exon 35, in order to detect SNP. A total of 11 synonymous single nucleotide polymorphisms (SNPs) were identified in the analysed sequence. After that the frequency of this 11 SNPs were verified in 2 Spanish sheep breeds with different production goal (Churra and Ojalada). The genetic variability of the gene is higher that other candidates analysed in sheep with a SNP every 420 bp. Five of the polymorphisms displays a high informativity in both populations and one of them is breedspecific (SNP09 in Churra). The characterization of the allelic variants reported herein laid the basis for further association analyses needed to evaluate the potential use of these SNPs as genetic markers for fat content and fatty acid composition of sheep products.
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