Micropropagation of Mangifera indica L. cv. Kurakkan through somatic embryogenesis
2010
Mishra, Maneesh | Shree, Yukti | Pati, Rajesh | Seal, Shubhendu | Shukla, Neelam | Kamle, Madhu | Ramesh Chandra | Srivastava, Alka
Nucellar embryogenesis was induced in Mangifera indica L. cv. Kurakkan, a polyembryonic salt tolerant, dwarfing rootstock. Nucellus tissue excised from 3.5 cm long fruits developed pro-embryonic callus in 19 days of inoculation on modified MS medium supplemented with 4.521JM 2,4 D, 0.05% malt extract and 13.781JM spermidine. Somatic embryogenesis exhibited high frequency (158.33 embryos). However, all the differentiated embryos proliferated on medium having low level of sucrose (4% w/v) and auxin (2.26IJM 2,4-0). Most of the proembryonic calli converted into heart shaped and cotyledonary embryos by reducing temperature to 15°C. Somatic embryos were matured on modified MS medium fortified with 0.381JM ABA, 0.571JM IAA and 30.30IJM PEG. Matured somatic embryos germinated (around 30%) on MS medium supplemented with 2.681JM NAA, 11.601JM kinetin and 2736.91JM glutamine.
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Эту запись предоставил Indian Council of Agricultural Research