Histoenzymic distribution pattern in liver of buffalo fetuses during prenatal development

Singh, Opinder; Kumar, Anoop

Histoenzymic distribution pattern in liver of buffalo fetuses during prenatal development

2009

Singh, Opinder | Kumar, Anoop

Histoenzymic distribution of oxidoreductases and phosphatases was studied in liver of buffalo fetuses ranging from 5 cm to 75 cm CRL (crown rump length). The succinic dehydrogenase (SDH), lactic dehydrogenase (LDH), reduced nicotinamide adenine dinucleotide diaphorase (NADHdiaphorase), reduced nicotinamide adenine dinucleotide Figs A_F: A. Liver of buffalo fetus of 44.5 cm CRL showing NADPH activity in hepatocytes surrounding the sinusoids. Nitro BT method×70. B. Cryosection of liver from 28.0 cm CRL buffalo fetus showing SDH activity in parenchyma. Nitro BT method×140. C. Cryosection of 1iver from 70.0 cm. CRL buffalo fetus showing weak MAO activity in parenchyma. Nitro BT method×70. D- Liver of buffalo fetus of 22.5 cm CRL showing strong AKPase activity in parenchyma of liver. Azodye method×140. E. Section of liver of from 70.0 cm CRL buffalo fetus showing strong ACPase activity in the parenchyma. Azodye method×70. F. Section of liver of from 22.5 cm CRL buffalo fetus showing moderate G_6_Pase activity in liver parenchyma. Lead nitrate method×70. D A E B F phosphate diaphorase (NADPH-D) and monoamine oxidase (MAO) exhibited variable activity in parenchyma of liver which may be attributed to developing histoarchitecture and activity of liver. Since the hemopoiesis took place during early prenatal period, the hemopoitic areas were weekly reactive. The activity of alkaline phosphatase, acid phosphatase and glucose_6_phosphatase was also recorded.

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