Study on the genetic variability of section Liseola in the Genus Fusarium , using morphological (VCGs) and molecular markers in Iran.
2008
Momeni, Hassan | Razavi, Mohammad | Kalanaki, Sirus | Zare`, Rasul
F. verticillioides is one of the main fungal pathogens of corn. It is a major pathogen of corn ear rot in Iran. This fungus causes root and stalk rot in many parts of the world. During 2005 isolates of the fungus were collected from different parts of the country. Nash-Snyder and PDA media were used to isolate the fungus. Purification of isolates was done using single-spore and hyphal-tip methods. VCG studies carried out including the generation of mutants, determination of nit type, completing tests and finally identification of vegetative compatibility groups. Fourteen VCG groups were obtained during this study. Random amplified polymorphic DNA (RAPD) analysis was used to assess genetic variation among 61 isolates of Fusarium verticillioides isolated from the main corn growing areas of five provinces (Ardabil, Fars, Mazandaran, Khorasan, and Khuzestan) in Iran. Nineteen RAPD haplotypes were also detected by analyzing 114 polymorphic bands. Seven random primers, OPR11, OPR14, OPR15, OPR19, OPR20, OPA3, and OPA4 revealed polymorphism among the isolates of this fungus. DNA bands were scored by assigning "0"for absence and "1"for presence and used in a matrix to compute genetic distance values among isolates. Comparison of RAPD classification with geographical factor revealed that geographical distribution of isolates is not consistent with RAPD classification. Cluster analysis with UPGMA using genetic distances showed that the isolates were of two main groups. These results suggested that RAPD is an effective marker for determination of diversity among isolalates of Fusarium verticillioides.
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