Detection of Different Iranian Isolates of Bovine Herpes Virus Type-1 (BHV-1) Using Polymerase Chain Reaction
2003
Kargar Moakhar, R., Razi Vaccine and Serum Research Institute,Tehran, Iran | Ghorashi, S.A., National Research Center for Genetic Engineering and Biotechnology,Tehran, Iran | Sadeghi, M.R., Veterinary School of Bu-Ali Sina University, Hamedan, Iran and Veterinary Medicine of Research Center of Markazi Province Arak, Iran, | Morshedi, D., National Research Center for Genetic Engineering and Biotechnology,Tehran, Iran | Masoudi, S., National Research Center for Genetic Engineering and Biotechnology,Tehran, Iran | Pourbakhsh, S.A., Diagnosis Dept., Razi Institute Received
Based on primers from gI glycoprotein, a polymerase chain reaction (PCR) assay was optimized for detection of bovine herpes virus type 1 (BHV-1). A 468bp DNA fragment of nine isolates was amplified. The PCR products were detected by ethidium bromide staining after gel electrophoresis and confirmed by sequencing. The nucleotide sequence alignments revealed a highly conserved region in gI gene within the isolates. Results suggest that PCR can be used as a reliable diagnostic tool for rapid detection of BHV-1 infections.
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