Bactericidal effect of chlorine dioxide on bacteria collected from removed implants
2010
Kuroyama, I., Nippon Dental Univ., Tokyo (Japan). School of Life Dentistry at Tokyo | Osato, S. | Nakajima, S. | Kubota, R. | Kaneko, Y.
We examined the bactericidal effects of chlorine dioxide (ClO2) on the bacteria collected from implant fixtures that were removed in two cases for peri-implantitis (inflammatory type) and overloading (overloading type) after 20 and 10 years from implant placement, respectively. The bacteria used in this study were from black (B) - and white (W) - colored colonies which proliferated in the inflammatory and overloading cases after 48-hour-anaerobic incubation on trypticase soy (TS) with horse whole blood agar medium. Porphyromonas gingivalis (P. gingivalis) was employed as a control. Four types of sterilized implant fixtures were contaminated by each bacterium, and then immersed in ClO2 only and activated ClO2 including 0.2, 0.5, 0.7 and 1.0% citric acid for 30 sec., 1, 3, 5 and 10 min. The bactericidal effects were assessed by the turbidity of the test tubes. The bactericidal effect against bacteria B (genus Porphyromonas) was seen in all materials within 5 minutes with 0.7% activated ClO2. Similar effects against bacteria W (genus Prevotella) and P. gingivalis appeared in 10 minutes with 0.7% activated ClO2. Ultrasonic cleaning using 0.2% activated ClO2 could kill bacteria on all the fixtures within 3 minutes. In this study, it was suggested that the technique using ultrasonic cleaning and 0.2% activated ClO2 would be useful as a strategic option for peri-implant tissue management.
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