A laboratory method for rearing tea green leafhopper, Empoasca onukii Matsuda by using tea seedlings as oviposition sites and as food
2010
Kosugi, Y., Shizuoka Prefectural Agriculture and Forestry Coll., Iwata (Japan)
In this study, I have developed a method for rearing tea green leafhopper, Empoasca onukii Matsuda by using tea seedlings. Tea seeds were prepared by incubating tea seeds at 5degC for 30 days and planted these seeds in agar gel. The germination rate of the seedlings on the 28th postincubation (at 25degC) days was 89.5%. When the length of new shoots was 7-8 cm, the seedlings were replanted in a cup containing agar gel. The cups were covered with a plastic cap and used as a rearing case. One adult female was captured from the tea fields and reared on the rearing case. On an average, 34.7 nymphs were found to breed on this rearing case. However, when the number of adult females in the rearing case was increased, the number of nymphs tended to decrease. Rearing of 1-2 adult males and females resulted in the breeding of more than 20 nymphs, ranging from the first to the fifth generation.
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