Extraction Efficiency and Stability of Anthocyanin Pigments in Black Soybean Seed Coat
2008
Kim, S.Y. (Soonchunhyang University, Asan, Republic of Korea) | Ko, K.O. (Soonchunhyang University, Asan, Republic of Korea) | Lee, Y.S. (Soonchunhyang University, Asan, Republic of Korea) | Kim, H.S. (Soonchunhyang University, Asan, Republic of Korea) | Kim, Y.H. (Soonchunhyang University, Asan, Republic of Korea), E-mail: [email protected]
This study was carried out to develop the methods for efficient extracting and stabilization of anthocyanin pigments in black colored soybean. The optimum solvent concentration for the highest pigment extraction from seed coat of black colored soybean was 60% MeOH with 0.1% HCl in laboratory test. The higher temperature of the solvent until 60℃ led to better extraction efficiency of anthocyanin. The anthocyanin extracts maintained almost its stability in strong acidity area whereas it destroyed markedly over pH 4. Also anthocyanin extracts was stable for a while under weak light condition, but it showed rapid degradation of pigment color and markedly decreased HPLC value for anthocyanin content after one month storage days. In case storage temperature and instrument, glass vessel on low temperature (4℃) was good storage condition among some treatments. And C3G was more stable than other pigments.
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