Effect of CO2 enrichment on in vitro plant regeneration through somatic embryogenesis in cyclamen (Cyclame persicum mill)
2010
Takamura, T, Kagawa Univ., Miki (Japan). Faculty of Agriculture | Imose, Y. | Tanaka, M.
The effect of CO2 enrichment (3000 ppm) on somatic embryogenesis in cyclamen was investigated. No significant difference between the growth of callus under CO2 enriched and normal conditions was observed. However, the calli induced under a CO2 enriched condition formed the largest number of somatic embryos after subculture under the same CO2 condition. The effect of CO2 enrichment on in vitro germination of somatic embryos was also examined. Larger number of plantlets with leaves and roots were observed in somatic embryos cultured under a CO2 enriched condition than under a natural condition. These results suggest the possibility of CO2 enrichment being one of the useful treatments for in vitro plant regeneration through somatic embryogenesis in cyclamen.
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