Paenibacillus pinihumi sp. nov., a cellulolytic bacterium isolated from the rhizosphere of Pinus densiflora
Kim, B.C., Biological Resources Center, KRIBB, Daejeon, Republic of Korea | Lee, K.H., Biological Resources Center, KRIBB, Daejeon, Republic of Korea | Kim, M.N., Biological Resources Center, KRIBB, Daejeon, Republic of Korea | Kim, E.M., Biological Resources Center, KRIBB, Daejeon, Republic of Korea | Rhee, M.S., Biological Resources Center, KRIBB, Daejeon, Republic of Korea | Kwon, O.Y., Chungnam National University, Daejeon, Republic of Korea | Shin, K.S., Biological Resources Center, KRIBB, Daejeon, Republic of Korea
A novel cellulolytic bacterium, strain S23∨T, was isolated from the rhizosphere of the pine trees in Daejeon, Republic of Korea. This isolate was Gram-positive, strictly aerobic, rod-shaped, catalase-negative, oxidase-positive, motile by means of peritrichous flagella, and tested positive for alkaline phosphatase, esterase lipase, leucine arylamidase, α-galactosidase, and β-galactosidase activities. The DNA G+C content was 49.5 mol%. The main cellular fatty acids were anteiso-C∧15:0 (51.9%), iso-C∧16:0 (14.7%), and iso-C∧15:0 (13.2%). The major isoprenoid quinone was menaquinone 7 (MK-7). Diagnostic diamino acid in the cell-wall pepti-doglycan was meso-diaminopimelic acid. Comparative 16S rRNA gene sequence analysis showed that this strain clustered with Paenibacillus species. The 16S rRNA gene sequence similarity values between S23∨T and other Paenibacillus species were between 89.9% and 95.9%, and S23∨T was most closely related to Paenibacillus tarimensis SA-7-6∨T. On the basis of phylogenetic and phenotypic properties of strain S23∨T, the isolate is considered as a novel species belonging to the genus Paenibacillus. Therefore, the name, Paenibacillus pinihumi sp. nov., is proposed for the rhizosphere isolate; the type strain is S23∨T (=KCTC 13695∨T =KACC 14199∨T =JCM 16419∨T).
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