Expression of Human Heavy-Chain and Light-Chain Ferritins in Saccharomyces cerevisiae for Functional Foods and Feeds
2008
Han, H.S. (Kyung Hee University, Yongin, Republic of Korea) | Lee, J.L. (Kyung Hee University, Yongin, Republic of Korea) | Park, S.H. (Kyung Hee University, Yongin, Republic of Korea) | Kim, J.H. (Kyung Hee University, Yongin, Republic of Korea) | Kim, H.Y. (Kyung Hee University, Yongin, Republic of Korea), E-mail: [email protected]
To produce human ferritins in yeast, human H-chain and L-chain ferritins were amplified from previously cloned vectors. Each amplified ferritin gene was inserted into the pYES2.1/V5-His-TOPO yeast expression vector under the control of the GAL1promoter. Western blot analysis of the recombinant yeast cells revealed that H-and L-chain subunits of human ferritin were expressed in Saccharomyces cerevisiae. Atomic absorption spectrometry (AAS) analysis demonstrated that the intracellular content of iron in the ferritin transformant was 1.6 to 1.8-fold higher than that of the control strain. Ferritin transformants could potentially supply iron-fortified nutrients for food and feed.
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