Adipocyte Culture Medium Stimulates GITRL Expression in MDA-MB-231 Cells
2009
Baek, A.M., Sookmyung Women's University, Seoul, Republic of Korea | Park, M.Y., Sookmyung Women's University, Seoul, Republic of Korea | Park, J.S., Sookmyung Women's University, Seoul, Republic of Korea | Han, J.H., Sookmyung Women's University, Seoul, Republic of Korea | Yang, Y., Sookmyung Women's University, Seoul, Republic of Korea
Little is known about roles of adipose tissue, although obesity is one of the potential risk factors in causing breast cancer and adipose tissue surrounding breast ductal cells is the largest organ. To identify the genes that are regulated by factors secreted from adipocytes in breast cancer cells, MDA-MB-231 cells were treated with the culture medium of adipocytes. In present study glucocorticoid-induced TNF receptor-related protein ligand (GITRL) gene was studied among the induced genes. It was found that GITRL was significantly increased by the culture medium of adipocytes. Proteinase K-treated adipocyte culture supernatants failed to induce GITRL expression. These findings indicate that unknown protein factors are responsible for the induction of GITRL expression. The expression of GITRL did not affect the invasive ability of MDA-MB-231 cells, but coculture with NK92 expressing GITR suppressed the invasiveness of MDA-MB-231 cells.
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