Nucleotide sequence similarity of the N genes among five Korean rabies live vaccine strains
2009
Yang, D.K. (National Veterinary Research and Quarantine Service, Anyang, Republic of Korea), E-mail: [email protected] | Kim, B.H. (National Veterinary Research and Quarantine Service, Anyang, Republic of Korea) | Lee, K.W. (National Veterinary Research and Quarantine Service, Anyang, Republic of Korea) | Choi, S.S. (National Veterinary Research and Quarantine Service, Anyang, Republic of Korea) | Son, S.W. (National Veterinary Research and Quarantine Service, Anyang, Republic of Korea) | Song, J.Y. (National Veterinary Research and Quarantine Service, Anyang, Republic of Korea)
Rabies, one of the oldest fatal zoonoses, is caused by rabies virus(RV) and has been reported in most parts of the world. In order to improve quality control of RV vaccine, the present study was conducted to investigate the nucleotide sequence similarity of partial nucleoprotein(N) gene derived from the commercially available five RV vaccines in Korea. The vero cells infected with rabies vaccine strains showed specific cytopathic effects, which was characterized by the rounding of cells. Three commercial RV vaccine strains had identical nucleotide and amino acid sequences. The other two RV vaccine strains showed one point mutation in amino acids of the N gene compared with those of ERA strain. Alignment with nucleotide sequences showed 99.9 to 100% similarity among five commercial vaccine strains. However, RV vaccine strains had low nucleotide similarity ranging from 90.1 to 90.7% with the Korean RV isolates, SKRBV9801YC and SKRBV0404HC, respectively. These results indicate that the strain, currently used in live rabies vaccine was derived from ERA strain as based on the nucleotide and deduced amino acid similarities.
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