Extract of Diospyros kaki Stem Reduces Neuronal Damage in the Gerbil Hippocampus Induced by Transient Cerebral Ischemia
2009
Yoo, K.Y., Hallym University, Chuncheon, Republic of Korea | Li, H., Hallym University, Chuncheon, Republic of Korea | Park, O.K., Hallym University, Chuncheon, Republic of Korea | Lee, C.H., Hallym University, Chuncheon, Republic of Korea | Choi, J.H., Hallym University, Chuncheon, Republic of Korea | Won, M.H., Hallym University, Chuncheon, Republic of Korea | Pai, Tongkun, Anyang University, Anyang, Republic of Korea | Hwang, I.K., Seoul National University, Seoul, Republic of Korea | Kim, J.D., Kangwon National University, Chuncheon, Republic of Korea
Diospyros kaki (Persimmon) fruits and leaves have been used as a traditional medicine and include a number of therapeutic components. The present study investigated neuroprotective effect of the extract of Diospyros kaki stem (EDKS) against ischemic damage in the gerbil hippocampal CA1 region after 5 min of transient cerebral ischemia. To elucidate the neuroprotective effect of EDKS on ischemic damage, 50 mg/kg EDKS was administered orally to gerbils for 7 days before the ischemic surgery, and we used cresyl violet and Fluoro-Jade C (F-J C) staining. In the EDKS-treated group, many survived pyramidal neurons in the CA1 region was stained with cresyl violet and a few degenerative pyramidal neurons was stained with F-J C. The survival rate of the CA1 pyramidal neurons was about 62% compared to that in the vehicletreated group. In the EDKS-treated group, isolectin B4 (a marker for activated microglia) immunoreactivity was significantly lower than that in the vehicle-treated group. These results suggest that repeated supplement of EKDS can protect neurons from ischemic damage.
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