Transplant Establishment of Pimpinella brachycarpa in Photomixotrophic and Photoautotrophic Culture System
2009
Na, H.Y., National Institute of Horticultural and Herbal Science, RDA, Suwon, Republic of Korea | Chun, C.H., Seoul National University, Seoul, Republic of Korea
Pimpinella brachycarpa somatic embryos were cultured photoautotrophically at different CO₂ concentrations, and conversion of the somatic embryos was compared to those cultured photomixotrphically. The growth rate of plantlet in photoautotrophic system did not show significant difference with that of plantlet in photomixotrophic system. While somatic embryo at torpedo stage didn't germinate at all at CO₂ concentration 400, 1,500 and 4,500 μmol mol-¹, the germination rate at CO₂ concentration 7,500 μmol mol-¹ was 31.3%. The germination rate of somatic embryos in cotyledonary torpedo stage increased as CO₂ concentration rate increased, showing that high concentration of CO₂ increases the photoautotrophic ability. When transplanting plants germinated at photomixotrophic system to ex vitro, the survival rate at three days after ex vitro was 0%. All explants withered in 24 hours. However, the survival rate of plants germinated at photoautotrophic system after transplanting to ex vitro was 100%, and it was possible to transplant without acclimatization process. Photoautotrophic system was applicable to P. brachycarpa for development of micropropagation system using somatic embryos being provided with the CO₂ concentration properly.
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