Diversity of non-starter lactic acid bacteria in Latvian semi-hard cheeses
2009
Mikelsone, A., Latvia Univ. of Agriculture, Jelgava (Latvia) | Ciprovica, I., Latvia Univ. of Agriculture, Jelgava (Latvia)
The most non-starter lactic acid bacteria (NSLAB) isolated from semi-hard cheeses are heterofermentative and handled as one of the reasons of cheese off-flavours and yield defect but at the same time majority of researches argued positive effect of NSLAB on cheese flavour formation and diversification due to rendered compounds of chemical reactions. The amount of NSLAB in cheese varies from 10 at the beginning of ripening to 105 cfu mlE-1 within 6-8 weeks. The aims of this paper were to establish diversity of NSLAB in commercial samples of Krievijas and Holandes cheeses, and to evaluate the effect of ripening temperatures on NSLAB in the trials of Krievijas cheese. A total of 12 commercial cheese samples from seven different Latvian manufacturers and trials from one cheese manufacturer were examined. The trials were ripened for 60 days at different temperatures - 6 deg C and 12 deg C. Serial dilutions of each cheese sample (1:1000 and 1:10 000) in saline were made. NSLAB were cultivated using MRS media. Strain identification was performed by the API 50 CHL system (BioMerieux, Marey l'Etoile, France). In commercial samples of Krievijas cheese dominance of L. curvatus was observed, simultaneously L.plantarum and L.paracasei subsp. paracasei were isolated. Whereas in Holandes cheese samples dominance of L.paracasei subsp. paracasei was noted and L.plantarum, L.curvatus, L.rhamnosus and L.acidophilus were isolated. In the trials ripened at different temperatures prevalence of L.curvatus was noted. Concentration of Lactobacillus spp. varied from 104 cfu mlE-1 on the first day of ripening and reached the highest concentration (106 cfu mlE-1) after 6 weeks of ripening.
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