Radiosensitivity of three species of ground orchids (Spathoglottis plicata, S. kimballiana var. angustifolia and S. tomentosa) to acute gamma radiation
2008
Gonzales, M.A., Central Luzon State Univ., Science City of Munoz, Nueva Ecija (Philippines). Coll. of Agriculture | Aurigue, F.B., Philippine Nuclear Research Inst., Quezon City (Philippines). Atomic Research Div. | Tapic, R.T., Central Luzon State Univ., Science City of Munoz, Nueva Ecija (Philippines). Coll. of Agriculture
A radiosensitivity study coupled with tissue culture technique was conducted as preliminary to mutation breeding of the three species of ground orchids (Spathoglottis plicata, S.kimballiana var. angustifolia, and S.tomentosa). It aimed to compare the effect of dose levels of gamma radiation applied to the germinated embryos (protocorms) of the three species. Also, it sought to determine the lethal dose and optimum dose of gamma radiation on the three species. The protocorms of the three species were irradiated at 10 Gy, 20 Gy, 30 Gy, 40 Gy, and 50 Gy dose level of gamma radiation. The three species have varied radiosensitivity as affected by their individual phenotype. Results showed that as the dose level and ministered increases, percent mortality of seedlings also increases whereas, the seedlings height, number of roots and root length decreased. However, there was an increase in the number of leaves at 10 and 20 Gy dose levels due to the emer`gence of furcations, but further increase in the dose levels of radiation decreased the number of leaves. Furthermore, some qualitative characters such as albinism, pigmentation, forked leaves, furcations, and multiple branching came out as responses to gamma irradiation.
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