Mapping of quantitative trait loci (QTL) for grain cooking and eating quality using a doubled haploid (DH) population from anther culture of indica/japonica hybrids of rice (Oryza sativa)
2010
Lapitan, V.C., Philippine Rice Research Inst., Los Baños, Laguna (Philippines). Plant Breeding and Biotechnology Div. | Redoña, E.D. | Brar, D.S., International Rice Research Inst., Los Baños, Laguna (Philippines). Plant Breeding, Genetcs and Biotechnology Div. | Abe, T., Yamagata Univ., 1-23 Wakaba-machi Yamagata 997-8555 (Japan). Dept. of Bioresource Engineering
Grain quality if becoming a selection criteria in most rice breeding programs. Twenty-four rice cultivars carrying good quality traits were evaluated for genetic diversity using SSR markers. Based on Shannon's diversity index, an overall genetic diversity of 0.71 was revealed indicating a high level of genetic variation among these cultivars. Two contrasting genotypes, PSB Rc10 (Indica) and Nipponbare (japonica), were identified as parents in the development of doubled haploid (DH) lines to constitute a mapping population thru anther culture. Morphological observations and SSR analysis revealed that they are valuable genetic resource for mapping of genes/QTLs for grain quality traits. The mapping population consisting of 219 DH lines and 205 SSR markers were used to identify the quantitative trait loci (QTL) for amylose content (AC), gel consistency (GC), and gelatinization temperature (GT). Thirteen QTLs were identified -three for AC and five each for GC and GT. Eight QTLs had major effects from which seven coincided with the most important loci identified in previous reports. All QTLs for AC were shown to have major effects on GC and GT. The QTL with the largest effect which accounted for 74% of the AC variation corresponded to the waxy (Wx) locus and a major QTL specifying GT was detected within the alkali degeneration (AlK locus. The identification of ATLs associated with these 3 traits and the SSR markers that have tight linkage to them may be used to speed up the process of breeding new rice varieties with better quality through marker-aided selection (MAS).
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