Manganese effects on in vitro development of lesser centaury (Centaurium pulchellum (Sw.) Druce)
2009
Todorovic, S., Institute for Biological Research Sinisa Stankovic, Belgrade (Serbia) | Giba, Z., University of Belgrade, Belgrade (Serbia). Faculty of Biology, Institute of Botany | Simonovic, A., Institute for Biological Research Sinisa Stankovic, Belgrade (Serbia) | Bozic, D., Institute for Biological Research Sinisa Stankovic, Belgrade (Serbia) | Banjanac, T. | Grubisic, D., University of Belgrade, Belgrade (Serbia). Faculty of Biology, Institute of Botany
To determine the manganese requirement necessary for optimal development of lesser centaury (Centaurea pulchellum) in vitro, we investigated the effect of exogenously applied Mn on different developmental process such as growth, flowering, fruiting, and seed germination. The application of Mn had no effect on stem length, except at the highest concentration of 10E-2 M, which was inhibitory. In addition, C. pulchellum plants were capable of in vitro flowering and fruiting even on media without added Mn. However, Mn content in the media affected seed dimensions, since both length and width of the seeds increased with increasing Mn concentration. Moreover, both excess and absence of Mn in the media caused appearance of necrotic plants. Exogenously applied Mn had no effect on seed germination percentage, except at concentrations greater than 3x10E-3 M.
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