RAPD analysis of genetic diversity and qualitative assessment of hydrolytic activities in a collection of Bacillus sp. isolate
Beric, T., University of Belgrade, Belgrade (Serbia). Faculty of Biology | Urdaci, M.C., Laboratoire de Microbiologie et Biochimie Appliquee, Gradignan (France) | Stankovic, S., University of Belgrade, Belgrade (Serbia). Faculty of Biology | Knezevic-Vukcevic, J., University of Belgrade, Belgrade (Serbia). Faculty of Biology
Genetic diversity and production of hydrolytic enzymes of 205 Bacillus isolates from different geographical and ecological niches in Serbia were studied. Combining RAPD analysis and 16S DNA sequencing, we determined 13 different groups of RAPD profiles within four (five) species: Bacillus subtilis, B. cereus/B.thuringiensis, B. pumilus, and B. firmus. Screening for production of hydrolytic enzymes showed that there was no correlation of enzyme production with species. Most of the isolates from all habitats produced amylase, protease, lipase, mannanase, and xylanase to some extent at 25 deg C and 37 deg C. The number of isolates that retained enzyme production ability at 55 deg C is considerably lower and they predominantly came for manure.
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