Comparison of two sample preparation procedures for HPLC determination of ochratoxin A
2009
Vukovic, G.L., Institute of Public Health, Belgrade (Serbia) | Pavlovic, S.Dj., Institute for Medicinal Plant Research Dr Josif Pancic, Belgrade (Serbia) | Ristic, M.S., Institute for Medicinal Plant Research Dr Josif Pancic, Belgrade (Serbia)
In this paper, the performance of two different clean-up procedures, viz., use of immunoaffinity and SPE columns, were compared, for determination of ochratoxin A in maize flour. The first method consisted of liquid-liquid extraction with a mixture of chloroform and phosphoric acid, followed by ion-exchange cleanup on Waters Oasis MAX columns. The second method consisted of extraction with a mixture of water and methanol, followed by LCTech OtaCLEAN immunoaffinity column cleanup. The both methods comply with present regulations but, the MAX sample clean-up procedure should be used as an alternative, since the immunoaffinity column clean-up procedure is characterized by better reproducibility, accuracy, and efficiency.
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