Comparative analysis of laboratory and field procedures for testing genetic purity of sunflower parent lines | Uporedna analiza genetske cistoce roditeljskih linija suncokreta u polju i laboratoriji
2010
Taski-Ajdukovic, K., Institut za ratarstvo i povrtarstvo, Novi Sad (Serbia) | Radic, V., Institut za ratarstvo i povrtarstvo, Novi Sad (Serbia) | Jevtic, A., Institut za ratarstvo i povrtarstvo, Novi Sad (Serbia) | Canak, P., Institut za ratarstvo i povrtarstvo, Novi Sad (Serbia) | Vujakovic, M., Institut za ratarstvo i povrtarstvo, Novi Sad (Serbia) | Miklic, V., Institut za ratarstvo i povrtarstvo, Novi Sad (Serbia)
One of the main features that confer high quality to the seed is its genetic purity. Genetic impurities in sunflower seed lot can originate from seed due to self-pollination, uncontrolled cross-pollination or mechanical mixing of seeds. Genetic purity of 18 seed lots of sunflower parental lines is controlled by comparative analysis of morphological traits and electrophoretic analysis of 4 isoenzyme systems, according to the UPOV guidelines for DUS test of sunflower lines and hybrids. In 83.3% of the total analysed samples, results in the field and laboratory were in agreement. In parental line R-8 5% genetic impurity was found by isoenzyme analysis, which was not detected based on morphological characteristics, while electrophoretic analysis of samples L-10 and L-11 showed less genetic impurities in comparison with the analysis in the field.
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