In vitro evaluation of boron tolerance in wheat (Triticum aestivum L.) genotypes
Kondic-Spika, A., Institute of Field and Vegetable Crops, Novi Sad (Serbia) | Kobiljski, B., Institute of Field and Vegetable Crops, Novi Sad (Serbia) | Marjanovic, M., Institute of Field and Vegetable Crops, Novi Sad (Serbia) | Hristov, N., Institute of Field and Vegetable Crops, Novi Sad (Serbia)
Wheat tolerance to high boron concentrations was determined using mature embryo culture. The testing was performed on a modified MS nutrient medium to which boric acid was added in two concentrations: 15 mM and 30 mM. The control medium contained no excess boric acid. The experiment involved 14 Serbian varieties. After one month of cultivation callus fresh weight (CFW) was measured and reductions of fresh callus weight (RFCW) at boron (B) concentration of 15 mM in relation to the control were calculated. The genotypes differed significantly according to their reaction to different B concentrations. Six genotypes had RFCWs below 50.0%, while eight genotypes had RFCWs above 50.1%. Cultivars Nevesinjka and Pesma were considered the most tolerant genotypes, while cultivars Balada and Vila were the most sensitive. The results confirmed that differences in genotype reactions to excess B are visible at the cellular level and that they may serve as a selection criterion.
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