In vitro synergistic antibacterial activity of Salvia officinalis L. and some preservatives
Stanojevic, D., University of Kragujevac, Kragujevac (Serbia). Faculty of Science | Comic, Lj., University of Kragujevac, Kragujevac (Serbia). Faculty of Science | Stefanovic, O., University of Kragujevac, Kragujevac (Serbia). Faculty of Science | Solujic-Sukdolak, S., University of Kragujevac, Kragujevac (Serbia). Faculty of Science
The aim of this study was to investigate the antibacterial activity of aqueous extracts of the species Salvia officinalis L. and its synergistic action with the preservatives sodium nitrate, sodium benzoate and potassium sorbate in vitro against selected food spoiling bacteria. Synergism was assessed by the checkerboard assay method and quantitatively represented by the FIC index. Synergistic action was established for aqueous extract/sodium benzoate, aqueous extract/potassium sorbate, and aqueous extract/sodium nitrate combinations. Synergism was detected in relation to: Agrobacterium tumefaciens, Bacillus subtilis, and Proteus sp. Synergism was established at plant extract and preservative concentrations corresponding up to 1/8 MIC values.
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