In vitro shoot regeneration from seedling explants in Brassica vegetables: red cabbage, broccoli, Savoy cabbage and cauliflower
Pavlović, S., Institute for Vegetable Crops, Smederevska Palanka (Serbia) | Vinterhalter, B., Institute for Biological Research Sinisa Stankovic, Belgrade (Serbia) | Mitić, N., Institute for Biological Research Sinisa Stankovic, Belgrade (Serbia) | Adžic, S., Institute for Vegetable Crops, Smederevska Palanka (Serbia) | Pavlović, N., Institute for Vegetable Crops, Smederevska Palanka (Serbia) | Zdravković, M., Institute for Vegetable Crops, Smederevska Palanka (Serbia) | Vinterhalter, D., Institute for Biological Research Sinisa Stankovic, Belgrade (Serbia)
Brassica oleracea varieties (red cabbage, broccoli, Savoy cabbage, and cauliflower) were tested for their ability to regenerate shoots in vitro. Cotyledon, hypocotyl and root explants of 7-day old seedlings were incubated on Murashige and Skoog (MS) medium supplemented with 1 mg/l 6-benzyladenine (BA) or 6-furfurylaminopurine (KIM) in combination with 0, 0.1, and 0.2 mg/l indole-3-butyril acid (IBA). Hypocotyls showed the best explants in almost all varieties tested with a minimum regeneration potential of 75% and producing 3.5-7.4 shoots per explant. The BA-supplemented media were optimal for both shoot regeneration and multiplication. Shoots rooted maximally (100%) on plant growth regulator-free MS medium containing 2% or 4% sucrose. Increased sucrose content improved plant acclimation in the greenhouse.
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