Genetic diversity of Austrian pine (Pinus nigra Arnold) populations in Serbia revealed by RAPD
2010
Lučić, A., Institute of Forestry, Belgrade (Serbia) | Mladenović-Drinić, S., Maize Research Institute Zemun Polje, Belgrade - Zemun (Serbia) | Stavretović, N., University of Belgrade, Belgrade (Serbia). Faculty of Forestry | Isajev, V., University of Belgrade, Belgrade (Serbia). Faculty of Forestry | Lavadinović, V., Institute of Forestry, Belgrade (Serbia) | Rakonjac, Lj., Institute of Forestry, Belgrade (Serbia) | Novaković, M., University of Belgrade, Belgrade (Serbia). Faculty of Forestry
The ability of random amplified polymorphic DNA (RAPD) to distinguish among populations of Austrian pine from Serbia was evaluated. Ten arbitrary 10mer primers employed in the analysis produced 113 fragments of which 100 were polymorphic (88.5%). All populations had specific RAPD phenotypes. Certain rare and genotype specific bands were identified which could be effectively used to distinguish the populations. Polymorphism RAPD markers among Pinus nigra population was high and sufficient to distinguish each of the populations. The results obtained suggest that RAPD markers are valuable for the genetic diversity estimation in Pinus nigra and for the study of the divergence among populations.
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