Analysis of secondary structure within sgm and kgmB mRNA
Vojnović, S., Institute of Molecular Genetics and Genetic Engineering, Belgrade (Serbia) | Ilić-Tomić, T., Institute of Molecular Genetics and Genetic Engineering, Belgrade (Serbia) | Morić, I., Institute of Molecular Genetics and Genetic Engineering, Belgrade (Serbia) | Vasiljević, B., Institute of Molecular Genetics and Genetic Engineering, Belgrade (Serbia)
Sgm methyltransferase from Micromonospora zionensis and Kgm B methyltransferase from Streptoalloteichus tenebrarius are resistant to aminoglycoside antibiotics as a result of their ability to specifically methylate G1405 within the bacterial 16S rRNA A-site. The (C)CGCCC motif, assumed to be a regulatory sequence responsible for the autoregulation of the sgm gene, could most likely also be responsible for the autoregulation of the kgmB gene. This sequence, found within the 5' untranslated region of both sgm and kgmB mRNAs, as indicated by in silico prediction, may be involved in the formation of a specific stem-loop structure. Sgm and KgmB are mutually down-regulated and it is likely that they share the same cis-acting elements. Structure probing experiments confirmed the existence of a stable secondary within the 5' UTR of the sgm mRNA, while the analysis of kgmB mRNA failed to confirm the predicted structure.
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