Determination of tea polysaccharides in Camellia sinensis by a modified phenol-sulfuric acid method
Xi, X., Shanghai Normal University, Shangai (China). College of Life and Environment Science | Wei, X., Shanghai Normal University, Shangai (China). College of Life and Environment Science | Wang, Y. | Chu, Q., Shanghai Normal University, Shangai (China). College of Life and Environment Science | Xiao, J., Shanghai Normal University, Shanghai (China). College of Life and Environment Science
A direct procedure for the determination of total polysaccharides (TPS) in Camellia sinensis was set up based on the modified phenol-sulphuric acid method. The monosaccharide composition of TPS was analyzed by GC. Based on the results of GC, model monosaccharide mixtures were made which provided an adequate standard for this procedure. Through single-factor and orthogonal experiments, the experimental conditions such as the volume of phenol, the volume of concentrated sulfuric acid, the reaction time, and the incubation temperature, were optimized. The highest sensitivity of absorbance was obtained when the volume of concentrated sulfuric acid, the volume of phenol (6%), and the incubation temperature were 2.5 ml, 0.2 ml, and 50 deg C, respectively. Under optimum conditions, the prepared samples were determined satisfactorily, with the recovery from 100.2% to 103.7%, and relative standard deviation (RSD) of 2.1%. Overall, the modified method is easily operated, rapid, sensitive and accurate. A similar procedure can be applied to the determination of other plant polysaccharides as well.
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