About the use of isolated embryo culture for obtaining of tulip hybrid forms
2009
Akhmetova, A.Sh. ([email protected]) | Bajburina, R.K. | Mironova, l.N., Russian Academy of Sciences. Ufa Science Centre (Russian Federation). Botanical Garden-Inst.
Embryos from seed of tulip hybrid forms were tested. The hybrids were a result of 5 breeding combinations of 9 varieties. The total number of the inoculated embryos was 140. The seeds were sterilized by 70% ethanol solution during 30 sec and by 0.2% diacid solution during 240 sec. Chloramin B could be used instead of diacid during 120 sec. The optimal period of embryo isolation was the 53-56 days after pollination when it was possible to pull out embryos of 1.5-3.0 milli m. At that case 91% of embryos started regenerating. The most intensive shoot proliferation was observed on the mediums containing 6-BAP (3.00 milli gram/l) and naphthylacetic acid (NAA) (0.15 mg/l). After 7 months an average number of shoots per an explant was 5.4 while shoot length was 0.043 m. The shoots elongated up to 0.050-0.055 m after 10 months. Cytokines were excluded from the medium and NAA was replaced by indolebytic acid (0.5 mg/l) for stimulating microbulb forming. Cultivating at dark at 4 deg C during 12 weeks inducted bulb-forming shoots. Then the regenerates were placed at light and were exposed at 26 deg C till the complete forming of microbulbs (30-120 days). The maximal output of microbulbs (39.9%) was at the hybrid combination Alladin x Blue heron; at the other combinations it was from 10.9 to 16.1%. The output of microbulbs was 6-8 units per an explant. The conclusion was made that the culture of isolated embryos allowed to keep and to propagate tulip hybrid forms effectively.
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