Biogenic amines production by bacteria isolated from poultry skin | Produkce biogenních aminů bakteriemi izolovanými z povrchu drůbeže
2009
Buňková, L., Univerzita Tomáše Bati ve Zlíně (Czech Republic) | Buňka, F. | Lukešová, P. | Mrkvička, V. | Doležalová, M. | Kráčmar, S.
The aim of this study is to explore production of biogenic amines (histamine, tyramine, putrescine, cadaverine, agmatine, spermine and spermidine) by 98 bacteria isolated from poultry skin (41 isolates of family Enterobacteriaceae, 21 isolates of genus Aeromonas, 16 isolates of genus Pseudomonas, 10 isolates of other gramnegative rods, and 10 isolates of gram-positive cocci). Ionexchange chromatography was employed to analyze the above mentioned amines. Enterobacteria were found the largest producers of amines with proved presence of tyramine, agmatine, putrescine, and cadaverine in cultivation broth after incubation of bacteria. Putrescine and cadaverine were the most abundant products. Presence of at least 2 biogenic amines, i.e. mainly concurrent presence of putrescine and cadaverine, was revealed in 19 enterobacteria strains. Eleven isolates classified into Aeromonas genus produced putrescine and five of them formed also cadaverine. Gram-positive cocci produced tyramine especially. The other observed biogenic amines (histamine, spermine and spermidine) were not found among tested isolates. Production of biogenic amines by any Pseudomonas family isolates was not proved.
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