Polyphenols and antioxidant activity of bee pollen | Polyfenoly a antioxidačná aktivita včelieho peľu
2009
Nôžková, J., Slovenská poľnohospodárska univ. v Nitre (Slovak Republic) | Fatrcová-Šramková, K. | Máriássyová, M. | Kropková, Z.
The objective of the study was to evaluate correlation between polyphenols content and antioxidant activity of selected bee pollen species: Brassica napus subsp. napus L., Helianthus annuus L. and Papaver somniferum L. The bee pollen samples were freeze-dried by table laboratory lyophilizator LYOVAC GT 2 by Amsco/Finn-Aqua. The process of lyophilization lasts until the samples reached 2 % moisture. Antioxidant activity was evaluated by DNABased biosensor, according to Heilerova et al. (2003). The polyphenols content was determined by spectrophotometer Folin-Ciocalteu agent with the tannin as reference standard (Singleton et al., 1999). The correlation analysis was evaluated by software SAS 9.1.3. Statistically significant correlation (P less than 0.05) between polyphenol content and antioxidant activity was determined. Higher values of contained polyphenol and antioxidant activity were detected in B. napus pollen versus H. annuus L. pollen (1.92-fold and 6.25-fold) and versus P. somniferum L. pollen (1.17-fold and 3.2-fold). The polyphenols content in studied samples was in the range from 799 to 1550 mg per kg. The antioxidant activity of bee pollen samples occurred in the range from 0.2 to 1.28. The antioxidant activity significantly differs in different plant species.
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