Structural changes in the testis caused by diazinon and selenium | Štrukturálne zmeny semenníkov po intraperitoneálnej aplikácii diazinonu a selénu
2010
Cabaj, M., Slovak Univ. of Agriculture in Nitra (Slovak Republic) | Toman, R., Slovak Univ. of Agriculture in Nitra (Slovak Republic) | Adamkovičová, M., Slovak Univ. of Agriculture in Nitra (Slovak Republic) | Massányi, P., Slovak Univ. of Agriculture in Nitra (Slovak Republic) | Šiška, B., Slovak Univ. of Agriculture in Nitra (Slovak Republic) | Lukáč, N., Slovak Univ. of Agriculture in Nitra (Slovak Republic) | Golian, J., Slovak Univ. of Agriculture in Nitra (Slovak Republic)
The aim of this study was to find the structural changes in the rat testis after a diazinon, selenium and their combined administration. The testis structural changes after the diazinon intraperitoneal administration of 20 mg per kg b.w., selenium 2 mg per kgb.w. and both diazinon 20 mg per kg b.w. and selenium 2 mg per kg b.w. were evaluated by histological and morphometric methods in light microscopy. 36 hours after the diazion i.p. administration, the vacuolization of the seminiferous epithelium, evacuation of germ cells into the tubule lumen, epithelium necrosis and disintegration, interstitium extension and fibrotization were observed. The germ cells released from the basal lamina and subsequently they were visible in the tubule lumen. Blood vessels were damaged and morphometric analysis have shown their significant dilatation (P less than 0.05). Diazinon causes the damage of the germinal epithelium in the testes leading to the spermatogenesis failure. The infertility can then appear. In selenium treated group disintegration of cellular associations in the seminiferous epithelium, damaged and separating spermatids lines, reduced spermatogenesis and significant vacuolization of seminiferous epithelium (P less than 0.0001) were observed. Similar changes as in selenium threated group occured, significant vacuolization of seminiferous epithelium (P less than 0.05) was seen. In combined diazinon and selenium treated group protective effects of selenium in seminiferous epithelium and interstitium were noticed. Further investigation of diazinon and selenium is needed to practical use this results.
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