Spectroscopic characteristics of humates isolated from different soil types
2009
Fasurová, N., University of Technology, Brno (Czech Republic) | Pospíšilová, Ľ., Mendel University of Agriculture and Forestry, Brno (Czech Republic)
In this project have been used the optical methods (UV-VIS and SFS). Five Czech soil humates samples (Leptic Cambisol, Haplic Cambisol, Eutric Cambisol 1-arable soil, Eutric Cambisol 2-grassland, Haplic Chernozem) were compared. Absorbance in spectral range 300-700 nm was measured using optical f ber on spectrometer Varian Cory 50 Probe. Fluorescence spectra (SFS) in the range 255-655 nm were measured by spectrofluorimeter Aminco Bowman. From UV-VIS spectra color indexes (Q4/6) were determined. Fluorescence indexes (F) were calculated from SFS spectra at DELTAlambda=55 nm as ratio RFI468/RFI522. The same main fluorophore at wavelengths lambdaex./ lambdaem.= 467/522 nm was observed in all soil humates. Samples were also compared by humus quality, carbon content and fluorescence or colour indexes.
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