Association of polymorphisms in the intron 1 of duck prolactin with egg performance
2009
Li, H.F., Chinese Academy of Agriculture Science, Institute of Poultry Science, Yangzhou (China) | Zhu, W., Chinese Academy of Agriculture Science, Institute of Poultry Science, Yangzhou (China) | Chen, K., Chinese Academy of Agriculture Science, Institute of Poultry Science, Yangzhou (China) | Zhang, T., Yangzhou University, College of Animal Science and Technology, Yangzhou (China) | Song, W., Yangzhou University, College of Animal Science and Technology, Yangzhou (China)
Gaoyou duck (Chinese indigenous breed) is famous for double-yolked egg. In the present study, SNP of prolactin (PRL) gene intron 1 of Gaoyou duck was detected by PCR-RFLP and DNA sequencing. The results indicated that there were 2 Dra I recognition sites in intron 1 of the PRL gene, but only 1 was of polymorphism. A T/C mutation at the position of the 1326 bp of the PRL gene was found by DNA sequencing, and resulted in 3 genotypes AA, AB, and BB. The frequencies of genotype BB and allele B were the highest. The association analysis between the polymorphism PRL gene intron 1 and egg performance was carried out. The least square analysis showed that the BB ducks had significant egg weight at the age of 30 weeks than AB ducks (P 0.01), the double-yolk percentage of the AB ducks was much higher than that of BB ducks (P 0.05) in double-yolk ducks, but there was not significant difference in egg number, longest clutch days, and the body weight at first egg among the three genotypes.
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