Quantitative trait loci influencing drought tolerance in rice (oryzsa sativa L.)
2009
Nguyen Thi Lang; Trinh Thi Luy; Bui Thi Duong Khuyeu; Nguyen Hoang Han, Bui Chi Buu
SSR technique combined with selective genotyping was used to map quantitative trait loci (QTLs) associated with tolerance for drought tolerance in rice. Two hundred twenty nine (BC2F2) derived from the cross between OM1490 /WAB880-1-38-18-20-P1-HB were evaluated. BC lines were evaluated for drought at flowering (DRF), dry root weigh (DR), root length (RL). Microsetellite map of this population was used with 232 markers to detect the linkage to target traits. A linkage map was constructed from 12-linkage groups based on the population. The map covers 2,553.7 cM with an average interval of 10.97 cM between marker loci. Markers associated with drought tolerance were located mostly on chromasomes 2, 3, 4, 8, 9, 10 and 12. Quantitative trait loci (QTL) mapping was used to determine effects of QTLs associated with drought tolerance traits. We also mapped QTLs for morphological attributes drought tolerance. Chi-square tests (÷2), single maker analysis (SMA), interval mapping (IM) were combined in QTL analysis procedure. All approaches are similar to QTL detection result. Fine QTLs were identified for DRR, two QTLs for length root, two QTLs for dry root weight. The proportion of phenotypic variation explained by each QTL ranged from 20.73% to 30.76% for DR, and from 6.23 to 3.39% for morphological characters at drought flowering. This study has provided much more detailed informations on the relative importance marker assiteced selection of drought tolerance.
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