EFFECT OF PENTOXIFYLLINE AND NEWLY SYNTHESIZED ORIGINAL METHYLXANTHINES ON FUNCTIONAL PARAMETERS OF ANIMAL SEMEN | Влияние на пентоксифилина и новосинтезирани оргинални метилксантини върху функционалните показатели на сперматозоидите при животните
2011
Petrov, P., Institute of Animal Science, Kostinbrod (Bulgaria) | Todorova, T., Institute of Animal Science, Kostinbrod (Bulgaria) | Todorov, P., Institute of Biology and Immunology of Reproduction "Acad. K. Bratanov", BAS, Sofia (Bulgaria) | Todorov, Ya., Institute of Animal Science, Kostinbrod (Bulgaria)
During cryopreservation a substantial reduction of motility and survival rates of spermatozoa is observed. This makes the issue of seeking effective stimulators of motility of the sperm very prospective. Big expectations in this regard are assigned to substances from the group of methylxanthines. The purpose of this study is to establish what is the impact of pentoxifylline and some original methylxanhines on motility of cryopreserved spermatozoa in order to establish the possibility for their practical use in the technology for artificial insemination of farm animals. In an experiment, methylxanthines - VZ1. VZ4. VZ5., etoxifylline and pentoxyfiline. were used in concentrations – 3.6 µmol/l and 7.2 µmol/l dissolved in culture media - medium199. sodium citrate. DMEM F-12. After thawing and subsequent dilution in mediums containing the methylxanhines to be tested they were incubated at 38°C and checked every 60 min. The newly synthesized methylxanthinesе designated VZ1 и VZ4 in concentration of 7.2 µmol/l showed the best results especially in the first hour or two.
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Эту запись предоставил Institute of Agricultural Economics