Effect of insulin-transferrin-selenium on goat oocytes maturation and embryo development
2008
Liu Fengjun | Zhang Yuling | Yang Zijun | Wang Guohua | Zhang Yong
[Objective]The research aimed to enhance culture efficiencies of oocyte and embryo of goat in vitro and explore serum-free culture system in vitro.[Method]At present, the conventional solutions of oocyte maturation and embryo development in vitro were always added into 1% ITS(Insulin-Transferrin-Selenium) or used 1% ITS to replace FBS in 2 kinds culture solutions for conducting in vitro cultures of goat oocyte and parthenogenetic embryo.The influences of ITS on their developments were detected.[Result] ITS in maturation liquid of oocytes could not increase oocytes maturation rate but significantly increased blastocyst rate (58.06% vs. 48.19%)of parthenogenetic embryo.If FBS in maturation liquid of oocytes was replaced by ITS, the maturation rate, cleavage rate and blastocyst rate were basically unchanged.Adding ITS into embryo medium could increase blastocyst rate (68.30% vs. 56.82%)of parthenogenetic embryo of goat. If FBS in embryo medium was replaced by ITS, the cleavage rate didn’t change basically, while the blastocyst rate in ITS was obviously lower than that in FBS group(42.33% vs.56.82%).[Conclusion] ITS could promote maturation of oocyte in vitro and early embryonic development, in addition, ITS could replace serum in maturation medium of oocyte as serum-free culture system for conducting relevant researches.
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