Cloning and prokaryotic expression of VP1 gene of foot-and-mouth disease virus (FMDV) type O
2008
Fu Wei | Chen Lei | Xiong Yi
According to the complete genome of foot-and-mouth disease virus (FMDV) type O, a pair of special primers was designed to amplify VP1 gene. The VP1 gene was amplified by RT-PCR and subsequently inserted into the expression vector pGEX-6p-1 and induced by IPTG. Then SDS-PAGE showed the expressed protein was 51 KD in molecular weight. Then the product was purified by GSTrap FF columns. The product was detected through Western-blot that showed the protein has antigenicity. It provided fundamental data and materials for further investigation on diagnosis method of FMDV.
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