Identification and pathogen characteristics analysis of a co-infection with CSFV, PRRSV and PCV2 | 猪繁殖与呼吸综合症•猪瘟和猪圆环病毒Ⅱ型混合感染的鉴定及病原特性分析
2010
Wu Jinyan, Chinese Academy of Agricultural Sciences, Lanzhou (China), Lanzhou Veterinary Research Institute | Tian Hong, Chinese Academy of Agricultural Sciences, Lanzhou (China), Lanzhou Veterinary Research Institute | Shang Youjun, Chinese Academy of Agricultural Sciences, Lanzhou (China), Lanzhou Veterinary Research Institute
[Objective] The aim of this study was to identify swine diseases caused by CSFV, PRRSV and PCV2 and thus to analyze its pathogeny chracteristics. [Method] The tissues and viscera of the diseased swine were collected from Xiangtan of Hunan (Code of HN/XT) to extract DNA and RNA for PCR amplification and sequencing. Meanwhile, the virulent strains were isolated and identified by cell separation technology. [Result] The sequencing analysis results showed that the amino acid homology between CSFV, PRRSV, PCV2 and sequences from current prevalent virulent strains or GenBank was about 90%, 94% and 96% respectively. Accordingly, the swine epidemic situation was caused by the mixed infection of three kinds of viruses (CSFV, PRRSV and PCV2) at least. [Conclusion] The research can provide data for the epidemiologic studies and purification control of the mixed infection diseases harmful for the swine breeding industry at present.
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