Extraction and content determination of shikimic acid in Pinus elliottii Engelm | 湿地松松针中莽草酸的提取及含量测定
2010
Xie Jiyun, Guangxi University, Nanning (China), College of Chemistry and Chemical Engineering | Chen Xiaopeng, Guangxi University, Nanning (China), College of Chemistry and Chemical Engineering | Li Zhirong, Guangxi University, Nanning (China), College of Chemistry and Chemical Engineering
[Objective] The aim was to provide scientific basis for development and utilization of Pinus elliottii Engelm resources. [Method] The extraction process of shikimic acid in Pinus elliottii Engelm was studied, and the content of shikimic acid was determined by HPLC. The HPLC conditions were as follows; Alltima NH2 (5um, 4.6mm × 150 mm) column separation; the mobile phase was acetonitrile-2%H3PO4 (90 : 10); the flowing velocity was 1 ml/min; test wavelength was 213 nm, the width of belt was 16 nm; reference wavelength was 300 nm, the width of belt was 80 nm. [Result] By the single factor and orthogonal tests, the optimum conditions were found as follows: ethanol concentration 60%, extraction temperature 75℃, solid-liquid ratio 1 : 25, extraction time 2.5 h. The extraction rate of shikimic acid was 1.49%. [Conclusion] Shikimic acid in Pinus elliottii Engelm could be used as a new resource to develop and utilize.
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