Studies on the isolation and screening of α-amylase producing strain and their enzymatic properties | α-淀粉酶产生菌分离筛选及酶学性质研究
2010
Liu Yaqin, North University for Nationalities, Yinchuan（China）, College of Biological Science and Engineering | Chen Haikui, North University for Nationalities, Yinchuan（China）, College of Biological Science and Engineering | Li Ruixue, North University for Nationalities, Yinchuan（China）, College of Biological Science and Engineering
[Objective] The aim was to obtain α-amylase producing strains with some excellent properties like high temperature resistance, strong acid resistance, strong alkali resistance, etc.. [Method] α-amylase producing strains were isolated and screened; furthermore their enzymatic properties were studied. [Result] 10 strains with an obvious starch hydrolysis cycle were screened out from starch screening plate coated by diluted sample, from which 3 strains with higher α-amylase activity were screened out, that was X6, X8 and X10. As for X6, X8 and X10, their optimum pH values all belonged to neutral, and their optimum temperatures were all 60 ℃. Meanwhile, Ca2+ could increase their enzyme thermal stability. When the concentration of Ca2+ was 0.02-0.04 mol/L, the enzyme thermal stability of X6 and X8 reached the highest; When the concentration of Ca2+ was 0.03-0.04 mol/L, that of X10 reached the highest; When the concentration of Ca2+ was increased continuously, those of the 3 strains all decreased. [Conclusion] The research provides theoretical basis for satisfying the demands of different industries for α-amylase with different characteristics.
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