Cloning and analysis of HN gene of Newcastle disease virus ND-xx08 isolate | 鸡新城疫病毒ND-xx08株HN基因的克隆及分析
2010
Jia Wenke, Henan Agricultural University,Zhengzhou(China), Engineering College of Animal Husbandry and Veterinary Science | Wang Xuannian, Henan Institute of Science and Technology,Xinxiang(China), College of Animal Science | Xu Lanju, Henan Agricultural University,Zhengzhou(China), Engineering College of Animal Husbandry and Veterinary Science
Китайский. 为克隆并分析新城疫病毒ND-xx08株HN基因,将其分离、纯化、培养,提取其病毒的基因组RNA,RT-PCR扩增获得与HN基因预期大小一致的DNA片段,克隆到pMD18-T载体,经EcoR I和Hind Ⅲ进行双酶切鉴定和测序鉴定.ND-xx08 HN基因的序列长度为1 716 bp,共编码571个氨基酸.与已发表的24株NDV HN序列的同源性分析表明,其核苷酸序列的同源性在80.0%~98.4%,氨基酸同源性在87.2%~98.2%.研究结果表明,ND-xx08具有强毒株的特性.[著者文摘]
Показать больше [+] Меньше [-]Английский. In order to clone and analyze of HN gene of Newcastle disease virus ND-xx08 isolate,it was separated,purified and cultured to obtain the virus genome RNA.DNA fragments was acquired by RT-PCR amplification.The DNA fragment was then cloned into pMD-18 vector for sequence and double enzyme digested by EcoR I and Hind III identification.The sequencing results showed the length of the HN gene is 1 716 bp,encoding 571 amino acids.The analysis showed that the nucleotide sequence homology was 80.0% to 98.4% and amino acid homology was 87.2% to 98.2% compared with 24 published NDV sequences.This research result indicated that ND-xx08 isolate had veolgenic strain characteristics.[著者文摘]
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