Antioxidative system in maize roots as affected by osmotic stress and different nitrogen sources
Vuletic, M.,Maize Research Inst., Zemun Polje (Serbia) | Hadzi-Taskovic Sukalovic, V.,Institute for Multidisciplinary Research, Belgrade (Serbia) | Markovic, K.,Maize Research Inst., Zemun Polje (Serbia) | Dragisic Maksimovic, J.,Institute for Multidisciplinary Research, Belgrade (Serbia)
The activities of antioxidative enzymes and contents of proline and total phenolics were assayed in roots of two maize genotypes grown in a medium containing nitrate (NO3−) or both nitrogen forms, nitrate and ammonium (NH4+/NO3−). An increase in the activities of class III peroxidases (POD), superoxide dismutase (SOD), ascorbate peroxidase (APX), ascorbate oxidase (AO) and proline content, and decrease in phenolic content were observed in NH4+/NO3− in comparison with NO3− grown plants. When polyethylene glycol (PEG) was added to both nitrogen treatments, the content of total phenolics and proline was increased, especially in NH4+/NO3− treatment. The PEG treatment decreased enzyme activities in NH4+/NO3− grown plants, but in NO3− grown plants activities of POD and SOD were increased, opposite to decreased APX and AO. Isoelectric focusing demonstrated increased activities of acidic POD isoforms in PEG treated NO3− grown plants, and lower activities of both, acidic and basic isoforms in NH4+/NO3− grown plants.
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