Isolation of resistance gene analogues to powdery mildew resistance sequences in hexaploid wheat
Feng, D.S.,Shandong Agricultural Univ., Tai'an (China). Agronomy Coll. | Ma, X.,Shandong Agricultural Univ., Tai'an (China). Agronomy Coll. | Lin, A.L.,Shandong Agricultural Univ., Tai'an (China). Coll. of Info and Science Engineering | Wang, H.G.,Shandong Agricultural Univ., Tai'an (China). Agronomy Coll. | Tian, J.C.,Shandong Agricultural Univ., Tai'an (China). Agronomy Coll.
This paper reports the characterization of the powdery mildew resistance homologous genes family of Triticum aestivum. Using degenerate primer pair for wheat resistance genes, we have cloned seven 3' truncated powdery mildew resistance gene homologous fragments Tpc5a, Tp25a, Tp25b, Tp3a5a, Tp3a5b, Tp4b5a and Tp4b5b. These fragments were sequenced. The deduced amino acid sequences showed that six of them have premature stop codons. All these sequences had a very high level of similarity to known Pm resistance genes such as Pm3a, Pm3b, Pm3d and Pm3f in hexaploid wheat. By ignoring the stop codons in the sequences, their deduced protein sequences were of coiled-coil (CC)-nucleotide binding site (NBS)-leucine repeat rich (LRR) structure. These results suggest that there are many powdery mildew resistance gene analogues in both resistant and susceptible wheat. Among them, small insertion/deletion events and point mutations can result in the diversity of wheat Pm resistance homologous genes.
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