In vitro regeneration of Solanum nigrum with enhanced solasodine production
Bhat, M.A.,Hamdard Univ., New Delhi (India). Dept. of Botany | Mujib, A.,Hamdard Univ., New Delhi (India). Dept. of Botany | Junaid, A.,Hamdard Univ., New Delhi (India). Dept. of Botany | Mahmooduzzafar,Hamdard Univ., New Delhi (India). Dept. of Botany
High frequency of shoot formation was achieved from Solanum nigrum leaves on Murashige and Skoog medium without any callusing stage. Shoot forming ability was more pronounced on leaves positioned dorsally. For shoot induction, 2.0 mg/cubic dm benzylaminopurine and 1.5 mg/cubic dm kinetin were observed to be the most effective plant growth regulators (PGRs). The present paper also describes first successful induction of in vitro flowering in S. nigrum. The leaf derived shoots were excised and treated with various root promoting PGRs and 0.25 mg/cubic dm indole-3-butyric acid produced maximum number of roots (15.2 per plant). Plants were later transplanted in field with 100 % survival. Solasodine content was higher in in vitro raised shoots and leaf derived callus in comparison with ex vitro grown shoots.
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